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Figure 1 | Microbial Cell Factories

Figure 1

From: Non-recombinant display of the B subunit of the heat labile toxin of Escherichia coli on wild type and mutant spores of Bacillus subtilis

Figure 1

Visualization of spore adsorption by western and dot blotting experiments. (A) Upon adsorption with purified LTB at various pH conditions, spore surface proteins were extracted by SDS-DTT treatment, fractionated on SDS-PAGE and analyzed by western blot. (B) The adsorption mixture (200 μl) was fractionated by centrifugation and different amounts of pellet (spores) and supernatant (free LTB) fractions were analyzed by dot blot. Serial dilutions of purified LTB were used as a standard. Immuno-reactions in A and B were performed with anti-LTB antibody [11] and anti-rabbit secondary antibody conjugated with the horseradish peroxidase.

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Microbial Cell Factories

ISSN: 1475-2859

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