Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae

Table 2 Antibiotic sensitivity testing

pH	Strain	G418 (μg/mL)				Phleomycin (μg/mL)
pH	Strain	200	400	600	800	20	40	60	80	100
5	S288C	+++	-	-	-	+++	+++	++	++	+
	EPY210C(pCEV-G2-Ph)	+++	-	-	-	+++	+++	+++	+++	+++
	EPY201C(pCEV-G2-Km)	+++	+++	+++	+++	+++	+++	++	++	+
7	S288C	+++	-	-	NT	++	-	-	NT	NT
	EPY210C(pCEV-G2-Ph)	+++	-	-	NT	+++	+++	+++	NT	NT
	EPY201C(pCEV-G2-Km)	+++	+++	+++	NT	++	-	-	NT	NT

Strains were streaked onto SD medium with pH adjusted to either 5 or 7, and supplemented with antibiotics at the concentrations noted. +++, strong growth, large colonies; ++, slow growth, small colonies; +, very poor growth in primary inoculum region only, no defined colonies; -, no growth. NT = not tested.

ISSN: 1475-2859