Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae

Table 1 Details for plasmid vectors

pCEV vector plasmid	Expression cassette 1		Expression cassette 2		Selection marker	GenBank accession
pCEV vector plasmid	Promoter	Unique RE sites	Promoter	Unique RE Sites	Selection marker	GenBank accession
pCEV-G1-Km	TEF1	NotI, SpeI, PacI	PGK1	BamHI, ApaI, ApaI, XmaI, SalI, Acc65I, KnpI, SacII, NheI	aphA1	KF366478
pCEV-G1-Ph	TEF1	NotI, SpeI, PacI	PGK1	BamHI, ApaI, HindIII, Acc65I, KnpI, SacII, NheI	ble	KF366479
pCEV-G2-Km	PGK1	NotI, SpeI, PacI	TEF1	BamHI, ApaI, ApaI, XmaI, SalI, Acc65I, KnpI, SacII, NheI	aphA1	KF366480
pCEV-G2-Ph	PGK1	NotI, SpeI, PacI	TEF1	BamHI, ApaI, HindIII, Acc65I, KnpI, SacII, NheI	ble	KF154123
pCEV-G3-Km	GAL10	SpeI, PacI	TEF1	BamHI, ApaI, ApaI, XmaI, SalI, Acc65I, KnpI, SacII, NheI	aphA1	KF366481
pCEV-G3-Ph	GAL10	SpeI, ClaI, PacI	TEF1	BamHI, ApaI, HindIII, Acc65I, KnpI, SacII, NheI	ble	KF366482
pCEV-G4-Km	HXT7	SpeI, PacI	TEF1	BamHI, ApaI, XmaI, SalI, Acc65I, KnpI, SacII, NheI	aphA1	KF366483
pCEV-G4-Ph	HXT7	SpeI, ClaI, PacI	TEF1	BamHI, HindIII, Acc65I, KnpI, SacII, NheI	ble	KF366484

Unique restriction sites are shown for commonly-found restriction enzymes (RE) in the multiple cloning site for each expression cassette (other unique sites are also present). Plasmid datasheets are available in the Additional file 1. Plasmid maps in Clone Manager format (Sci-Ed Software, http://www.scied.com) are available through the AddGene website (http://www.addgene.org/).

ISSN: 1475-2859