Table 3 Comparison of measured metabolites and calculated kinetic and stoichiometric parameters between three fermentations of strain AR36 with different substrate concentrations
Strain | AR36 | AR36 | AR36 |
Culture conditions | Batch 50 g/L Glc + 15 g/L YE | Batch 100 g/L Glc + 15 g/L YE | Batch 100 g/L Glc + 30 g/L YE |
SA titer (g/L) | 23.80 ± 0.00 | 41.80 ± 2.83 | 43.30 ± 0.57 |
Glc consumed (g/L) | 52.65 ± 1.20 | 103.70 ± 6.79 | 105.55 ± 4.45 |
Duration of culture (h) | 32 | 60 | 30 |
Y SA/Glc (mol/mol) | 0.47 ± 0.01 | 0.42 ± 0.00 | 0.42 ± 0.01 |
Acetate titer (g/L) | 1.45 ± 0.00 | 11.90 ± 0.14 | 8.65 ± 0.92 |
X max (g/L) | 6.30 ± 0.09 | 6.54 ± 0.09 | 12.54 ± 0.06 |
μ (h -1 ) | 0.53 ± 0.03 | 0.45 ± 0.02 | 0.45 ± 0.00 |
Qp global (gSA/L*h) | 0.74 ± 0.00 | 0.75 ± 0.07 | 1.44 ± 0.02 |
Qs global (gGlc/L*h) | -1.65 ± 0.04 | -1.74 ± 0.11 | -3.52 ± 0.15 |
qp exp (gSA/gDCW*h) | 0.38 ± 0.00 | 0.34 ± 0.03 | 0.46 ± 0.06 |
qs exp (gGlc/gDCW*h) | -1.14 ± 0.15 | -1.11 ± 0.00 | -1.18 ± 0.03 |
qp sta (gSA/gDCW*h) | 0.16 ± 0.01 | 0.19 ± 0.02 | 0.20 ± 0.01 |
qs sta (gGlc/gDCW*h) | -0.36 ± 0.01 | -0.42 ± 0.01 | -0.42 ± 0.03 |