Figure 2

Screen validation and hit identification. (A) The screen protocol was validated with the known HIV inhibitors Zidovudine (AZT), Enfuvirtide (T20) and Indinavir (IND). Values are plotted as % mean luciferase expression relative to solvent controls. (B-D) The results of the myxobacterial library screen are shown for Viability (B), infectivity Part 1 (C) and re-infection Part 2 (D). For each test compound, the % mean RLU of the control is plotted vs. the calculated robust Z-score. Hits are defined as compounds inhibiting HIV by at least 50% of the untreated controls (infectivity Z-score < 0) and with less than 30% compound-related toxicity (viability Z-score > 0) (blue boxes). Error bars are standard deviations of quadruple measurements.