Figure 2

Display of endoxylanase on cell surface. (a) SDS-PAGE analysis and (b) western blotting analysis of E. coli displaying BAN-fused XynA. Lane M, molecular weight size markers; Lanes 1, 2 and 3, E. coli host only without plasmid; Lanes 4, 5, and 6, E. coli harboring pTJ1-pelB-XynA; Lanes 7, 8, and 9, E. coli harboring pTJ1-BAN-XynA; Lanes 10, 11, and 12, E. coli harboring pTJ1-pelB-BAN-XynA. Lanes 1, 4, 7, and 10, total proteins; Lanes 2, 5, 8, and 11, periplasmic protein fraction; Lanes 3, 6, 9, and 12, outer membrane fraction. Arrows in lanes 5, 9 and 12 indicate XynA (~21 kDa), BAN-XynA with His6 tag (~27 kDa), BAN-XynA (~25 kDa) and, respectively. Closed and open arrowheads indicate the bands of OmpA and OmpC proteins in outer membrane proteins fraction, respectively.