Figure 6
Dynamics of Fab accumulation into periplasm and extracellular medium. Fab fragment F1 was expressed in E. coli RV308 and BL21(DE3) in the fed-batch medium and Ultra Yield shake flasks at 150 rpm shaking. Fab quantities in cell lysate (periplasmic fraction; solid line with black circle) and broth supernatant (medium fraction; dashed line with triangle) were measured by antigen-binding ELISA, and are shown at different time points as percentage of the final Fab yield (left vertical axis). Cell density was recorded by OD600 readings (dotted line with square), which are shown on the right vertical axis.