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Table 1 Fatty acid composition and accumulation in cellular lipids of Y. lipolytica strains, cultivated on YPD medium

From: Genetic engineering of Yarrowia lipolytica for enhanced production of trans-10, cis-12 conjugated linoleic acid

Fatty acid

Transformant

Polh-1312-o pai-8

Polh-1312-o pai-d12-5

Polh-1312-spo pai-10

Polh-1312-spo pai- d12-9

Polh-1292-spo pai- d12-7

Polh-1292-spo pai- d12-16

16:0

8.2 ± 0.4

11.2 ± 0.3

9.5 ± 0.6

11.8 ± 0.9

13.9 ± 0.7

13.3 ± 0.5

16:1

11.3 ± 0.7

6.4 ± 0.8

11.3 ± 1.2

10.5 ± 0.7

12.4 ± 0.8

10.7 ± 1.1

16:2

ND

2.6 ± 0.3

ND

1.0 ± 0.0

0.7 ± 0.1

0.4 ± 0.0

17:1

2.2 ± 0.2

2.3 ± 0.1

2.1 ± 0.1

2.4 ± 0.2

2.5 ± 0.2

2.4 ± 0.3

18:0

0.3 ± 0.0

1.3 ± 0.1

1.2 ± 0.0

1.3 ± 0.2

0.6 ± 0.0

1.1 ± 0.1

18:1

49.7 ± 2.0

13.2 ± 0.5

48.8 ± 1.6

25.9 ± 1.2

21.9 ± 0.8

26.4 ± 1.0

18:2

27.8 ± 1.1

62.2 ± 2.2

26.0 ± 1.3

44.8 ± 1.7

40.3 ± 0.9

35.9 ± 0.5

10 t, 12c-CLA

0.4 ± 0.1

0.7 ± 0.1

1.1 ± 0.2

2.2 ± 0.4

7.8 ± 0.7

9.8 ± 0.5

TFA (% of DCW)

4.2 ± 0.2

4.0 ± 0.1

4.8 ± 0.4

4.5 ± 0.2

3.7 ± 0.1

3.9 ± 0.3

  1. Values are presented as % (w/w) of the total fatty acid levels in yeast transformants. The means ± SD were obtained from three independent experiments. TFA, total fatty acid levels; ND, not detected.