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Figure 3 | Microbial Cell Factories

Figure 3

From: Genetic engineering of Yarrowia lipolytica for enhanced production of trans-10, cis-12 conjugated linoleic acid

Figure 3

Growth characteristics, lipid content and CLA production of Y. lipolytica Polh- 1292- sp opai- d12- 16 in batch fermentations with YNBD-SO medium. The best-performing strain, Polh-1292-spopai-d12-16, was cultivated in YNBD-SO medium for 168 hours under fermentation conditions. (A) The growth of Y. lipolytica was represented by lipid-free biomass calculated after subtraction of the cellular lipids from the total biomass. The glucose concentration was quantified using glucose oxidase in a standard glucose assay kit. (B), (C) Time course of lipid and CLA contents in cells and culture medium. Lipid and CLA yields in cells are expressed as percentage of total DCW. CLA titre in cells and medium and lipid titres in medium are expressed as g/l. The values represent the mean ± SD of three replicates.

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Microbial Cell Factories

ISSN: 1475-2859

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