Table 2 All investigated extraction solution and the downstream analytic used are noted for each extraction method
From: Methodological approaches to help unravel the intracellular metabolome of Bacillus subtilis
Extraction solution | Applied method |
|---|---|
Cooled methanol, water and chloroform mixture (4:2:4) | GC-MS and LC-MS |
Cooled water, 100% | LC-MS |
Boiling water, 100%: the filter including cells was added to a plastic | GC-MS |
Centrifuge tube and the sample were heated for 10 min in a 100°C water bath. | |
Cooled 60% (w/v) methanol | GC-MS |
Cooled 60% (w/v) ethanol | GC-MS and LC-MS |
Boiling 60% (w/v) ethanol, accomplished as for boiling water. | GC-MS and LC-MS |
Cooled 1 M formic acid | GC-MS |
Cooled 100% acetonitrile | GC-MS and LC-MS |
Cooled ethanol 60% (w/v) followed by a second water extraction | LC-MS |
Cooled ethanol 60% (w/v) followed by a second chloroform extraction | LC-MS |
Cooled ethanol 60% (w/v) followed by a second acetonitrile extraction | LC-MS |