Figure 6

Comparison of the extraction power of several tested extraction techniques, detected via LC-MS measurement. Y-axis values are not indicated, since all values are relative abundances related to the integral of the internal standard Br-ATP. F-1,6-P: fructose-1,6-bisphosphate and F-6-P/G-6-P: sum of the relative amount of glucose-6-phosphate and fructose-6-phosphate. A) ■ Light gray; cooled 60% ethanol, ▤ striped; mixture of methanol/water/chloroform, ■ dark gray; cooled water. B) ■ Light gray; cooled water, ▤ striped; two step extraction by first cooled 60% ethanol and a subsequent 100% chloroform extraction, ■ dark gray; two step extraction by first cooled 60% ethanol and a subsequent 100% water extraction. C) ■ Light gray; two step extraction by first cooled 60% ethanol and a subsequent 100% water extraction, ▤ striped; cooled 60% ethanol, ■ dark gray; liquid nitrogen cooling followed by a two step extraction by first cooled 60% ethanol and subsequent a 100% water extraction. D) ■ Light gray; 100% cooled acetonitrile, ▤ striped; liquid nitrogen cooling followed by a two step extraction by first cooled 60% ethanol and a subsequent 100% acetonitrile extraction, ■ dark gray; liquid nitrogen cooling followed by a two step extraction by cooled first 60% ethanol and a subsequent 100% water extraction.