Figure 8
From: A single mutation in the core domain of the lac repressor reduces leakiness
LacIWFhas increased stability in the presence of operator DNA. Purified LacI and LacIWF were incubated at a final concentration of 1 μM in the absence (grey) or presence of different concentrations of IPTG (brown and dark red for 1 mM and 10 mM, respectively) or operator DNA (2.5 μM, blue) and their unfolding temperatures measured by DSF. The increase in melting temperature caused by the presence of each ligand (ΔT) is reported above the corresponding bar.