Figure 7
From: A single mutation in the core domain of the lac repressor reduces leakiness
LacIWFreduces the cytotoxicity of a plasmid encoding a MBP-HIV-protease. A. Optical density of BL21(DE3) cell cultures bearing pLIGFP (blue line, open squares), pLIGFP_W220F (red line, open squares), pLIHP (blue line, solid circles) or pLIHP_W220F (red line, solid circles) in the absence of IPTG. B. Comparison of un-induced (filled circles) and IPTG-induced (open circles) cells expressing the MBP-HIV protease fusion under the control of LacI (light/dark blue) or LacIWF (dark/light red). Cell cultures were induced after 2 hours (black vertical line).