Figure 6From: Disruption of rimP-SC, encoding a ribosome assembly cofactor, markedly enhances the production of several antibiotics in Streptomyces coelicolorEffect of rimP-SC disruption on MetK expression. (A) Detection of MetK by western blotting analysis. (B) Transcriptional analysis of metK by real-time RT-PCR. (C) The production of actinorhodin. Cell cultures (50Â mg) at each time point were treated with KOH (final concentration, 1Â N) and the OD640 corresponding to 10Â mg of mycelium was determined. Data were presented as the averages of the results of three independent experiments in triplicate. Error bars showed standard deviations.Back to article page