Table 4 Primers used in this study
Primer | Application | Sequence (5′-3′) |
|---|---|---|
JSP10 | Amplification of arsB fragment (PmeI) | GGCGCAGCTGTTTAAAC GTCCTGACCATCGTATTGG |
JSP11 | Amplification of arsB fragment (PmeI) | GGGTCAGCTGTTTAAAC GTCCCAAATCGCAGCCAAT |
JSP22 | Screening oligo for KIKO vector inserts | TTCTGCGAAGTGATCTTCCG |
JSP55 | Amplification of lacZ fragment | GCGAGTTTAAAC CATTTTCCGTGACGTCTCGT |
JSP56 | Amplification of lacZ fragment (PmeI) | GCGAGTTTAAAC AAGACTGTTACCCATCGCGT |
JSP57 | Amplification of rbsAR fragment (PmeI) | GCGAGTTTAAAC CAAGCGGCATTGTGTATATC |
JSP58 | Amplification of rbsAR fragment (PmeI) | GCGAGTTTAAAC CCCAGTTCATCTTTCGGTTG |
JSP64 | Amplification of R6K ori and bla (PvuII) | TAGGCAGCTGG GAGGATATTCAAATGGACC |
JSP65 | Amplification of R6K ori and bla (PvuII) | CCCCCAGCTGG ATGCAGGTGGCACTTTTCG |
JSP123 | Confirmation of arsB genomic insertion | CAACCTGGCTCGACAAAACT |
JSP124 | Confirmation of arsB genomic insertion | GTGTCACAAACAGCACAGGC |
JSP125 | Confirmation of rbsAR genomic insertion | CCGAACTGATGAAAGTGCTC |
JSP126 | Confirmation of rbsAR genomic insertion | GCGTAAATCTAAGCCGAACC |
JSP129 | Confirmation of lacZ genomic insertion | GTCTGAATTTGACCTGAGCG |
JSP130 | Confirmation of lacZ genomic insertion | TCATACAGAACTGGCGATCG |
JSP148 | Amplification of xynA (PstI) | AGGACTGCAG CGGAGCTTATCGACTGCACG |
JSP149 | Amplification of xynA (SalI) | AAACGCTGAC TTAACGAGGAGCGGCAGAGG |
cscB_F2 | Screening oligo for cscAKB insertion | ATGGCACTGAATATTCCATTCAGA |
KO Test | Confirmation of insertion junction at Cm end | GGAGTGAATACCACGACGAT |