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Table 1 Direct comparison of integration using linearised plasmid and pcr products

From: Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

Locus Strain Template Colonies WT locus Single X Double X Background
arsB W Plasmid 38 33 2 3 92%
PCR 2 0 2 0 100%
MG1655 Plasmid 38 4 15 19 50%
PCR 14 0 2 12 14%
lacZ W Plasmid 8 5 1 2 75%
PCR 6 0 1 5 17%
MG1655 Plasmid 32 25 6 1 97%
PCR 39 5 12 22 44%
rbsAR W Plasmid 22 0 16 6 73%
PCR 7 0 5 2 71%
MG1655 Plasmid 8 0 8 0 100%
PCR 15 0 14 1 93%
  1. Templates for each transformation (linearised plasmid or PCR) are noted, as well as the number of colonies obtained from each transformation, the number of colonies having a wild type locus (no integration), the number of colonies having a single cross-over (Single X; integration of entire plasmid) and the number of colonies having a double cross-over (Double X; integration of only the region contained between the homologous arms). Background % is calculated based on the number of WT locus + Single X colonies.