Table 1 Gene functions related to the promoters used in this work (from the Saccharomyces Genome Database)
From: Repressible promoters – A novel tool to generate conditional mutants in Pichia pastoris
Gene | Function | Inducible/repressible |
|---|---|---|
THI11 | Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP) | Repressible with addition of thiamine |
THR1 | Conserved protein required for threonine biosynthesis (homoserine kinase) | Repressible with addition of L-threonine, L-valine, L-leucine and L-isoleucine |
MET3 | ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism | Repressible with addition of L-methionine |
SER1 | 3-phosphoserine aminotransferase, catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis | Repressible with addition of L-serine |
PIS1 | Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins | Repressible with addition of zink |