Table 2 List of primers used on this study
From: Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis
Name | Sequence | Restriction site |
|---|---|---|
GME-F | 5’CTCGAG ATGGGAACTACCAATGGAACAG3’ | XhoI |
GME-RFlag | 5’CCCGGCGGCCGTCA CTTGTCATCGTCATCCTTGTAATCCTCTTTTCCATCAGCCGCG3’ | NotI |
VTC2-F | 5’GCGGCCGC ATGTTGAAAATCAAAAGAGTTCCGACC3’ | NotI |
VTC2-RFlag | 5’AGGCCTTCA CTTGTCATCGTCATCCTTGTAATCCTGAAGGACAAGGCACTCGGCGGC3’ | StuI |
VTC4-F | 5’CTCGAG ATGGCGGACAATGATTCTCTAG3’ | XhoI |
VTC4-RFlag | 5’AGGCCTTCA CTTGTCATCGTCATCCTTGTAATCTGCCCCTGTAAGCCGC3’ | StuI |
VT4-F | 5’CGACTCGGTACC ATGGCGGACAATGATTCTCTAG3’ | KpnI |
VT4-R | 5’CGACTCGAATTCTCA CTTGTCATCGTCATCCTTG3’ | EcoRI |
hisG I – F | 5’TGTACA CCAGTGGTGCATGAACGC3’ | BsrGI |
hisG I – R | 5’ACATGT CTAGGGATAACAGGGTAATATAGACATGG3’ | BsrGI |
hisG II – F | 5’CGACTCCCCGGG CCAGTGGTGCATGAACGC3’ | XmaI/SmaI |
hisG II – R | 5’CGACTCCTGCAG CTAGGGATAACAGGGTAATATAGACATGG3’ | PstI |
KanMX-F | 5’CGACTCTGTACA CTGAAGCTTCGTACGCTGCA3’ | BsrGI |
KanMX-R | 5’CGACTCCCCGGG ATCACCTAATAACTTCGTATAGCATACATTATAC3’ | SmaI |
GPDADH1-F | 5’CGACTCCATATG GCGGCCGC GTCGAAACTAAGTTCTTGGTGTTTTAAAACT3’ | NdeI /NotI |
GPDADH1-R | 5’CGACTCGACGTC AAGCTT GGCATGCGAAGGAAAATGAGA3’ | AatII / HindIII |
KlACT1-F | 5’ATGGATTCTGAGGTCGCTGC3’ | |
KlACT1-R | 5’TTAGAAACACTTCAAGTGAACGATGG3’ | |
P1 | 5’ACACACGTAAACGCGCTCGGT3’ | |
P2 | 5’ATCATCCTTGTCAGCGAAAGC3’ | |
P3 | 5’ACCTGAAGATAGAGCTTCTAA3’ | |
P4 | 5’GGTACCCCTAGGAGATCTAGCTC3’ |