Figure 2
From: Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis
Genomic organization of SacII linearized vectors into the K. lactis LAC4 locus upon transformation. (A) Scheme of single and multiple copies of integrated GME cassettes detected by PCR using primers P1 and P3 (2.4 kb) or P3 and P4 (2.3 kb) respectively; (B) Scheme of single and multiple copies of AtVTC2 and AtVTC4 cassettes detected by PCR using primers P1 and P2 (2.4 kb) or P3 and P2 (2.3 kb) respectively.