Table 1 Carbon balance of HMS174, RV308, and BL21 cultivations for the entire process and the separate process phases (non-induced phase, production phase, and end phase)
HMS174 | Experiment (0 to 28Â h) | Phase I (0 to 7Â h) | Phase II (7 to 16Â h) | Phase III (16 to 28Â h) | |||||
|---|---|---|---|---|---|---|---|---|---|
| Â | Â | g | % | g | % | g | % | g | % |
Glucose provided | 1095.0 | 50.6 | 135.3 | 100.0 | 197.9 | 91.1 | 761. 5 | 31.3 | |
Glucose consumed | 553.8 ± 31.83 | 135.3 ± 022 | 180.4 ± 0.93 | 238.1 ± 31.83 |  |  |  |  | |
C in consumed glucose | C6H12O6 | 201.4 ± 11.58 |  | 49.2 ± 0.08 |  | 65.6 ± 0.34 |  | 86.6 ± 11.32 |  |
 C in biomass | CH1.77O0.49 N0.24 | 28.5 ± 0.23 | 14.2 | 21.4 ± 0.20 | 43.4 | 7.2 ± 0.05 | 10.9 | non-detectable | |
 C in acetate | CH3COO- | 8.9 ± 0.64 | 4.4 | non-detectable | non-detectable | 8.9 ± 0.68 | 10.2 | ||
 C in formate | HCOO- | non-detectable | non-detectable | non-detectable | non-detectable | ||||
 C in pyruvate | C3H3O3 | 2.4 ± 0.10 | 1.2 | non-detectable | 0.1 ± 0.00 | 0.2 | 2.3 ± 0.09 | 2.7 | |
 C in carbon dioxide | CO2 | 86.6 ± 2.77 | 43.0 | 23.1 ± 1.31 | 46.9 | 34.7 ± 1.88 | 52.9 | 28.9 ± 0.53 | 33.4 |
Sum of C detected | 126.5 | 62.8 | 44.4 | 90.3 | 41.9 | 64.0 | 40.1 | 46.3 | |
C not detected | 74.8 | 37.2 | 4.8 | 9.7 | 23.6 | 36.0 | 46.5 | 53.7 | |
RV308 | Experiment (0 to 28Â h) | Phase I (0 to 7Â h) | Phase II (7 to 16Â h) | Phase III (16 to 28Â h) | |||||
| Â | Â | g | % | g | % | g | % | g | % |
Glucose provided | 1103.6 | 100.0 | 135.3 | 100.0 | 197.1 | 100.0 | 771.3 | 100.0 | |
Glucose consumed | 1103.6 ± 0.00 |  | 135.3 ± 0.00 |  | 197.1 ± 0.00 |  | 771.3 ± 0.00 |  | |
C in consumed glucose | C6H12O6 | 401.3 ± 0.00 |  | 49.2 |  | 71.7 |  | 280.5 |  |
 C in biomass | CH1.77O0.49 N0.24 | 152.5 ± 0.46 | 38.0 | 26.7 | 54.3 | 23.2 | 32.3 | 102.7 | 36.6 |
 C in acetate | CH3COO- | 0.3 ± 0.04 | 0.1 | non-detectable | 0.1 | 0.1 | 0.2 | 0.1 | |
 C in formate | HCOO- | 0.4 ± 0.07 | 0.1 | non-detectable | 0.1 | 0.1 | 0.3 | 0.1 | |
 C in pyruvate | C3H3O3 | non-detectable | non-detectable | non-detectable | non-detectable | ||||
 C in carbon dioxide | CO2 | 190.1 ± 3.51 | 47.4 | 19.6 ± 0.21 | 39.9 | 40.5 ± 1.59 | 56.6 | 130.0 ± 2.24 | 46.3 |
Sum of C detected | 343.3 | 85.5 | 46.3 | 94.1 | 63.8 | 89.0 | 233.2 | 83.1 | |
C not detected | 58.0 | 14.5 | 2.9 | 5.9 | 7.9 | 11.0 | 47.3 | 16.9 | |
BL21 | Experiment (0 to 28Â h) | Phase I (0 to 7Â h) | Phase II (7 to 16Â h) | Phase III (16 to 28Â h) | |||||
| Â | Â | g | % | g | % | g | % | g | % |
Glucose provided | 1103.8 | 99.8 | 135.6 | 99.8 | 197.9 | 99.6 | 770.6 | 99.9 | |
Glucose consumed | 1101.6 ± 0.01 |  | 135.3 ± 0.03 |  | 196.8 ± 0.15 |  | 769.6 ± 0.11 |  | |
C in consumed glucose | C6H12O6 | 400.7 ± 0.00 |  | 49.2 ± 0.01 |  | 71.6 ± 0.05 |  | 279.8 ± 0.04 |  |
 C in biomass | CH1.77O0.49 N0.24 | 152.2 ± 2.02 | 38.0 | 24.9 ± 0.03 | 50.4 | 14.6 ± 0.46 | 20.4 | 112.7 ± 1.56 | 40.3 |
 C in acetate | CH3COO- | 0.6 ± 0.10 | 0.1 | non-detectable | 0.3 ± 0.04 | 0.4 | 0.3 ± 0.10 | 0.1 | |
 C in formate | HCOO- | 0.3 ± 0.09 | 0.0 | 0.1 ± 0.03 | 0.1 | 0.1 ± 0.04 | 0.1 | 0.2 ± 0.07 | 0.1 |
 C in pyruvate | C3H3O3 | 0.1 ± 0.10 | 0.0 | non-detectable | non-detectable | 0.1 ± 0.05 | 0.0 | ||
 C in carbon dioxide | CO2 | 216.5 ± 1.16 | 54.0 | 25.5 ± 0.54 | 51.8 | 48.2 ± 0.50 | 67.3 | 142.8 ± 1.26 | 51.0 |
Sum of C detected | 369.4 | 92.1 | 50.5 | 102.6 | 63.1 | 88.1 | 256.1 | 91.5 | |
C not detected | 31.3 | 7.9 | -1.3 | -2.6 | 8.5 | 11.9 | 23.8 | 8.5 | |