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Figure 1 | Microbial Cell Factories

Figure 1

From: Homofermentative production of optically pure L-lactic acid from xylose by genetically engineered Escherichia coli B

Figure 1

Engineering a xylose-to-L-lactic acid homofermentative pathway (1.2 mole xylose = > 2 mole L-lactic acid). Genes encoding important enzymes are indicated by italics. The relevant gene encoding enzymes are: pdh, pyruvate dehydrogenase complex (engineered for anaerobic expression in a previous study); pta, phosphotransacetylase; adhE, alcohol dehydrogenase; ackA, acetate kinase; ldhA, D-lactate dehydrogenase; ldhL, L-lactate dehydrogenase (expressed from ldhA promoter); pflB, pyruvate formate lyase; frdABCD, fumurate reductase. The abbreviated metabolic intermediates are: GAP, glyceraldehyde-3-phosphate; PEP, phosphoenol pyruvate. The stop sign indicated the relevant gene was deleted.

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Microbial Cell Factories

ISSN: 1475-2859

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