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Table 4 C. sonorensis ATCC32109 derived strains constructed and studied in this work

From: Production of l-lactic acid by the yeast Candida sonorensis expressing heterologous bacterial and fungal lactate dehydrogenases

Description

Transformed with plasmid(s)

pdc1∆

pMI267

pdc2∆

pMI287

pdc1∆ pdc2∆

pMI267, pMI287

pdc1∆::BmLDH

pMI265

pdc2∆::BmLDH

pMI286

pdc1∆::BmLDH pdc2∆

pMI265, pMI287

x::BmLDH

pMI265

x::BmLDH y::BmLDH

pMI265

x::LhLDH

pMI246

x::LhLDH

pMI247

x::LhLDH y::LhLDH

pMI257

x::LhLDH-LhLDH-LhLDH

pMI247

pdc1∆::LhLDH

pMI257

pdc1∆::RoLDH

pMI266

pdc1∆::LhLDH pdc2∆

pMI257, pMI287

pdc1∆::RoLDH pdc2∆

pMI266, pMI287

pdc1∆::LhLDH pdc2∆::LhLDH

pMI257, pMI288

pdc1∆::BmLDH pdc2∆::BmLDH

pMI265, pMI286

  1. The heterologous LhLDH, BmLDH, and RoLDH genes were expressed under the control of the C. sonorensis PGK1 promoter; the MEL5 and G418R marker genes were expressed under the C. sonorensis GPD1 or PGK1 promoter (not indicated in the table). x:: and y:: indicate that the site of integration is not known. Two consecutive transformations were made to construct a strain where two plasmids are listed.
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Microbial Cell Factories

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