Table 1 Overlapping sequences for homologous recombination
| Â | Comparison to S. cerevisiae CEN.PK113-7D genome | |||
|---|---|---|---|---|
SHR-sequence | Sequence 5’ ➔ 3’ | Blast score | E-value | % GC content |
Optimized SHR-sequences | Â | Â | Â | |
A | ACTATATGTGAAGGCATGGCTATGGCACGGCAGACATTCCGCCAGATCATCAATAGGCAC | 28.3 | 1.3 | 50.0 |
B | CACCTTTCGAGAGGACGATGCCCGTGTCTAAATGATTCGACCAGCCTAAGAATGTTCAAC | 30.1 | 0.37 | 48.3 |
C | ACGTCTCACGGATCGTATATGCCGTAGCGACAATCTAAGAACTATGCGAGGACACGCTAG | 26.5 | 4.5 | 50.0 |
D | ACGCATCTACGACTGTGGGTCCCGTGGAGAAATGTATGAAACCCTGTATGGAGAGTGATT | 28.3 | 1.3 | 48.3 |
F | CATACGTTGAAACTACGGCAAAGGATTGGTCAGATCGCTTCATACAGGGAAAGTTCGGCA | 28.3 | 1.3 | 46.7 |
G | GCCAGAGGTATAGACATAGCCAGACCTACCTAATTGGTGCATCAGGTGGTCATGGCCCTT | 28.3 | 1.3 | 51.7 |
H | AGATTACTCTAACGCCTCAGCCATCATCGGTAATAGCTCGAATTGCTGAGAACCCGTGAC | 30.1 | 0.37 | 48.3 |
I | TATTCACGTAGACGGATAGGTATAGCCAGACATCAGCAGCATACTTCGGGAACCGTAGGC | 28.3 | 1.3 | 50.0 |
J | GGCCGTCATATACGCGAAGATGTCCAAGCAGGTAGAACACATAGTCTGAGCATCTCGTCG | 26.5 | 4.5 | 51.7 |
Endogenous sequences | Â | Â | Â | |
A# | GTCGACAACCCTTAATATAACTTCGTATAATGTATGCTATACGAAGTTATTAGGTCTAGA | 28.3 | 1.3 | 33.3 |
B# | GAGTGTTTAGAACATAATCAGTTTATCCATGGTCTATCTCTTCTTGTCGCTTTTTCTCCT | 28.3 | 1.3 | 36.7 |
C# | TTAATTTTAAATTTTTTTGGTAGTAAAAGATGCTTATATAAGGATTTCGTATTTATTGTT | 109 | 5e-25 | 18.3 |
D# | TAATATTTTTTCTTTTGAAAGTACTACCCACATCCGAACATTGCCACTTACATAGCGATG | 109 | 5e-25 | 35.0 |
J# | GAACAAAGTATTTAACGCACATGTATAAATATTGTATTAAAAGGGTACCTTTATAAATAT | 109 | 5e-25 | 23.3 |
F# | GAATAGTCTTTACACCCACAGTTTTTCGTGTGGCAGTTACTATATATTAGTAGGATATTC | 109 | 5e-25 | 35.0 |
G# | CTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTT | 30.1 | 0.37 | 35.0 |
H# | ACGTAGGATTATTATAACTCAAAAAAATGGCATTATTCTAAGTAAGTTAAATATCCGTAA | 109 | 5e-25 | 25.0 |
I# | TTGGCAATTTTTTGCTCTTCTATATAACAGTTGAAATTTGAATAAGAACATCTTCTCAAA | 109 | 5e-25 | 26.7 |