Table 1 Peptides, plasmid and strains used in the study

HC124 (POI)

gsdpgipwwniraplnagagipwwniraplnaggsgpgsggntsqlstgggntsqlstggpkkpgdpgipwwniraplnagagipwwniraplnaggsgpgsggntsqlstgggntsqlstggpkkpgd

HC415 (POI)

gsdpsaqsqlpdkhsglherapqrygpeeaakkeeaakkpahinktnphqgnhhsektqrqgsggggsgsggggsdshhnhhkqdsrpqhrktpngggdshhnhhkqdsrpqhrktpngk

QC1100

MG1655 ΔaraBAD ΔslyD, expression host for POI

QC1101

QC1100 host expressing HC124 under pBAD promoter on pBR vector

QC1525

QC1100 host expressing HC415 under pBAD promoter on pBR vector

pDCQ601

Vector containing a linker of multiple cloning sites introduced at the EcoR I site downstream of the Pcat promoter on pBHR1 (MoBiTec, Germany)

RK1 library

BL21 genomic DNA fragments expressed in QC1101 producing HC124

QC3800 library

Mixture of Keio collection strains producing HC415

BW25113

Parental strain for the Keio collection

JW0710

BW25113 ΔgltA, Keio collection strain containing ΔgltA knockout

Colony 181

Isolate from RK1 library that contained the ysaB-glyQ-glyS locus