Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses

Table 2 Bacterial strains and plasmids used in this study

Strains	Containing genes	source
E. coli K12 MG1655	xylA, xylB, tktA, talB, yfdZ, metB	ATCC 47076
P. furious	Pfu-sHSP	ATCC 43587
Z. mobilis CP4(CP4)	Wild-type Z. mobilis	ATCC 31821
Z. mobilis XU(XU)	xylA, xylB, tktA, talB	This study
Z. mobilis HYM(HYM)	yfdZ, metB, Pfu-sHSP	This study
Z. mobilis HYMX (HYMX)	yfdZ, metB, Pfu-sHSP, xylA, xylB, tktA, talB	This study
Plasmids
pTN-HMY	Pfu-sHSP, metB, yfdz	This study
pTN-XU	xylA, xylB, tktA, talB	This study
pTN -HMYX	yfdZ, metB, Pfu-sHSP, xylA, xylB, tktA, talB	This study

yfdZ and metB genes were used for self-synthesizing lysine and methionine;
xylA, xylB, tktA and talB genes were used for utilizing xylose to produce ethanol;
Pfu-sHSP gene was used for increasing the tolerance to heat stresses.

ISSN: 1475-2859