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Figure 8 | Microbial Cell Factories

Figure 8

From: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses

Figure 8

Constructions of Tn5 transposon - pTN-HMY. Commercial EZ-Tn5 pMOD vector was mutated to create vector pTN by substituting the PvuI and PshAI with SfiI restriction site. To allow selection of Z. mobilis transformants after electroporation with the Tn5 transposon, tetracycline resistance determinant (TC) was cloned into the multiple cloning site in the vector pTN. The sHSP/metB/yfdZ operon was cloned into the SalI site of pTN to create pTN-HYM. This plasmid contains SfiI restriction site recognized sequences flanking the mosaic end (ME) sites, which are specifically recognized by the EZ-Tn5 transposase.

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Microbial Cell Factories

ISSN: 1475-2859

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