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Figure 11 | Microbial Cell Factories

Figure 11

From: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses

Figure 11

Generation of Tn5 transposon vector pTN-HYMX for random mutagenesis of Z. mobilis . The xylA/xylB/talB/tktA operon and sHSP/metB/yfdZ operons were cloned into the SalI site of pTN to create the plasmid pTN-HYMX. This plasmid contains SfiI recognized sequences flanking the mosaic end (ME) sites, which are specifically recognized by the EZ-Tn5 transposase.

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Microbial Cell Factories

ISSN: 1475-2859

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