Figure 10From: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocellulosesGeneration of Tn5 transposon vector pTN-XU for random mutagenesis of Z. mobilis . The xylA/xylB/talB/tktA operon for adding a metabolic pathway for Z. mobilis to utilize xylose was inserted into the SalI site to create pTN-XU. This plasmid contains SfiI recognized sequences flanking the mosaic end (ME) sites, which are specifically recognized by the EZ-Tn5 transposase.Back to article page