Table 1 Bacterial strains and plasmids used in this study

E. coli DH5α

F^-, ϕ80d, lacZ ΔM15, endA 1, recA 1, hsdR 17(r_K^-m_K^-), supE 44, thi-1, gyrA 96, relA 1, Δ(lacZYA-argF)U169

laboratory strain

E. coli JM109

F′ traD36 proA⁺B⁺lacI^qΔ (lacZ)M15/ Δ (lac-proAB) glnV44 e14^-gyrA96 recA1 relA1 endA1 thi hsdR17

laboratory strain

E. coli JM109 gwBC

E. coli JM109, rbsDK::manC, melAB::manB, xylAB::gmdwcaG

this study

E. coli JM109 gwBC-F1

E. coli JM109, rbsDK::manC, melAB::manB, xylAB::gmdwcaG, malEG::futC

this study

E. coli JM109 gwBC-F1-fkp

E. coli JM109, rbsDK::manC, melAB::manB, xylAB::gmdwcaG, malEG::futC, fucIK ::fkp

this study

E. coli JM109 gwBC-F2

E. coli JM109, rbsDK::manC, melAB::manB, xylAB::gmdwcaG, malEG::futC, fucIK ::futC

this study

E. coli JM109 gwBC-F2-fkp

E. coli JM109, rbsDK::manC, melAB::manB, xylAB::gmdwcaG, malEG::futC, fucIK::futC, araBAD::fkp

this study

Plasmids

pKD46

P_araB γ β exo (red recombinase), Amp^R

[35]

pCP20

FLP⁺, λ cI857⁺, λ p_R Rep^ts, Amp^R, Cm^R

[34]

pMJC54

pET16b, B. fragilis fkp gene, Amp^R

[17]

pCAW48

pET11a, S. enterica manB gene, Amp^R

Albermann & Piepersberg (unpublished)

pCAW49

pET11a, S. enterica manC gene, Amp^R

Albermann & Piepersberg (unpublished)

pCAW55

pJOE2775, H. pylori futC gene, Amp^R

[14]

pCAS30-FRT-cat-FRT

pJF119ΔN, P. ananatis crtE gene, FRT-sites, Amp^R, Cm^R

[36]

pJF-crtY-FRT-kan-FRT

pJF119ΔN, P. ananatis crtY gene, FRT-sites, Amp^R, Kan^R

[32]

pQE31-FRT-cat-FRT

pQE31, FRT-sites, Amp^R, Cm^R

[37]

pJF119ΔN

cloning vector, RBS, P_tac, Amp^R

[38]

pJF-manB

pJF119ΔN, S. enterica manB gene, Amp^R

this study

pJF-manC

pJF119ΔN, S. enterica manC gene, Amp^R

this study

pJF-gmdwcaG

pJF119ΔN, E. coli gmd, wcaG gene, Amp^R

this study

pJF-futC

pJF119ΔN, H. pylori futC gene, Amp^R

this study

pJF-fkp

pJF119ΔN, B. fragilis fkp gene, Amp^R

this study

pJF-manB-FRT-cat-FRT

pJF119ΔN, S. enterica manB gene, FRT-sites, Amp^R, Cm^R

this study

pJF-manC-FRT-kan-FRT

pJF119ΔN, S. enterica manC gene, FRT-sites, Amp^R, Kan^R

this study

pJF-gmdwcaG-FRT-cat-FRT

pJF119ΔN, E. coli gmd, wcaG gene, FRT-sites, Amp^R, Cm^R

this study

pJF-futC-FRT-cat-FRT

pJF119ΔN, H. pylori futC gene, FRT-sites, Amp^R, Cm^R

this study

pJF-fkp-FRT-cat-FRT

pJF119ΔN, B. fragilis fkp gene, FRT-sites, Amp^R, Cm^R

this study