Figure 1
From: Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production
Half-reactions of the FAD cofactor in the flavodehydrogenase domain of CDH. In the reductive half-reaction the oxidized FAD is reduced by e.g. cellobiose, which is converted into the 1,5-lactone before it spontaneously hydrolyzes in the aqueous reaction medium to the aldonic acid. The reduced FAD is regenerated in the oxidative half-reaction by electron acceptors or, very slowly, by oxygen.