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Table 4 UP are variably over-secreted relative to α-sec-EAP in the panel of E(M)

From: A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter

Lanes

Strain/colony/

GCB Levels of EAP & α-sec- EAP or HSA, in E(P),

Levels of UP1 in E(P),

Levels of UP3 in E(P),

 

Methanol (70 Hrs)

h-V, H & E(M)

E(M), h-V, H,

E(M), h-V, H,

(N)

(N)

(N)

2

EAP(0.08U)/lead edge

141.7591

0.0757

0.0197

3

E(P)

1

1

1

4

h-V

1.7566

0.5539

1.0984

5

H

279.1085

2.0422

1.4002

6

E(M9)-B

1.7995

0.6655

2.0953

7

E(M32)-A

8.8916

2.3354

1.8787

8

E(M32)-B

10.2686

0.9618

2.3738

9

E(M32)-C

11.5889

0.3545

2.0224

10

E(M32)-D

12.3115

0.4713

1.9284

11

E(M44)-A

57.8638

3.606

1.7049

12

E(M44)-B

56.309

3.9762

1.0414