Expression and functional studies of genes involved in transport and metabolism of glycerol in Pachysolen tannophilus

Table 1 Genes potentially involved in glycerol metabolism in P. tannophilus

Annotated function	Gene name *S. cerevisiae*	Gene name *P. tannophilus*	Size (bp)	Identity/Similarity	Accession no.
Transport
Glycerol Facilitator	ScFPS1	PtFPS1	1170	35/49	[GenBank:JQ481631]
		PtFSP2	972	32/51	[GenBank:JQ481632]
Glycerol Symporter	ScSTL1	PtSTL1	1728	34/51	[GenBank:JQ481633]
		PtSTL2	1905	31/50	[GenBank:JQ481634]
Consumption
Glycerol kinase	ScGUT1	PtGUT1	1848	53/67	[GenBank:JQ481635]
G3P dehydrogenase	ScGUT2	PtGUT2	1998	52/66	[GenBank:JQ481636]
Glycerol dehydrogenase	ScGCY0031	PtGCY1	936	54/73	[GenBank:JQ481637]
		PtGCY2	933	51/70	[GenBank:JQ481638]
Dihydroxyacetone kinase	ScDAK1	PtDAK	1767	42/58	[GenBank:JQ481639]
	ScDAK2
Production
G3P dehydrogenase	ScGPD1	PtGPD	1314	67/80	[GenBank:JQ481640]
	ScGPD2
G3-phosphatase	ScGPP1	PtGPP	705	33/53	[GenBank:JQ481641]
	ScGPP2

The potential genes were identified by performing a BLAST search with known S. cerevisiae genes as queries. Sequences were deposited in Genbank and the accession no. is listed in the table. Sizes given for P. tannophilus genes.

ISSN: 1475-2859