Table 2 Densitometric analysis
UreA source | Amount of protein used (ng) | Density in OD/mm2(standard deviation) | UreA concentration (ng) in extracts (% of total) | n° of recombinant molecules extracted from each spore |
|---|---|---|---|---|
Fusion 1 | ||||
Purified UreA | 25.0 ng | 177.8 (± 0.03) | NA | |
12.5 ng | 85,7 (± 0.09) | NA | ||
6.25 ng | 41.6 (± 0.08) | NA | ||
KH102 (CotZ-UreA) | 10.00 μg | 17,4 (± 0,03) | 2.44 (0.02) | 2.5 × 10 2 |
5.00 μg | 8,6 (± 0,01) | 1.20 (0.02) | ||
2.50 μg | 4,4 (± 0,08) | 0.61(0.02) | ||
Fusion 2 | ||||
Purified UreA | 25.0 ng | 121.4 (± 0.02) | NA | |
12.5 ng | 61,3 (± 0.02) | NA | ||
6.25 ng | 30.8 (± 0.05) | NA | ||
KH124 (CotB-linker -UreA | 1.250 μg | 67.2 (± 0.03) | 13.83 (1.10) | 1.0 × 10 4 |
0.625 μg | 34.5 (± 0.09) | 7.10 (1,14) | ||
0.312 μg | 15.6 (± 0.08) | 3.21 (1.04) |