Figure 11

Effect of acetic acid on growth of E. coli BL21 DE3(+)/ pET25b/ SELP- 59- A. Effect of acetic acid, added at an elapsed fermentation time of 0 hours, on growth of E. coli BL21 DE3(+)/pET25b/SELP-59-A cultivated under the optimised shake flask conditions of the present study. The variation in the initial growth rates (measured over the initial 30 minutes of incubation) as a function of the amount of acetic acid added (top), and biomass levels (as measured by g/L DCW) over the course of the bioprocess (bottom) are shown.