Table 1 Bacterial strains and plasmids used for this study
Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
Strains | ||
R. eutropha | ||
 H16 | Wild type | DSM 428, ATCC 17699 |
 H16-KLB4 | H16 transformed by pEDY-KLB4 | This study |
E. coli | ||
 XL-1 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacIq lacZΔM15 Tn10 (Tcr)] | Stratagene Cloning Systems Inc. |
 S17-1 | Tra+recA pro thi hsdR, chr::RP4-2 | 21 |
 HB101 | F-, leuB6 Δ(gpt-proA)62 recA13 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL20(Str+) supE44 Δ(mcrC-mrr) | Takara Bio Inc. |
Plasmids | ||
pBluescript KS (+) | Aprlac Z’, T7 gene 10 promoter, f1 ori | Stratagene Cloning Systems Inc. |
pSE-KLB2 | 1,183-bp Eco RI-Afl II fragment containing the complete KlADH gene in pSE420 | 15 |
pCH591 | 260-bp Hin dIII-Nde I fragment containing the ReSH promoter in Litmus 29 | 25 |
pEDY309 | RK2 ori, Tcr, Mob+ | 25 |
pBlueKLB3F | 401-bp PCR fragment containing a part of KlADH gene in Eco RV digested pBluescript KS (+) | This study |
pCH-KLB2 | 385-bp Nde I-Xba I fragment from pBlueKLB3F in pCH591 downstream of ReSH promoter | This study |
pCH-KLB3 | 1,005-bp Pfl MI-Xba I fragment from pSE-KLB2 in pCH-KLB2 | This study |
pEDY-KLB4 | Eco RV-Sna BI fragment containing a complete set of the ReSH promoter and KlADH gene from pCH-KLB3 in Swa I digested pEDY309 | This study |