Figure 4
Influence of P H2 on the H 2 -driven reaction by R. eutropha transformant with a jar fermenter. The reaction mixture (120 ml) contained 110 ml cell suspension of H16-KLB4 (1 g wet cell/5 ml reaction buffer) and 2% (v/v) hydroxyacetone. (A) As the figure shows, the PH2 supplied to the reaction mixture decreased from 100% to 10% in a stepwise manner. The SF prepared from the cell suspension used for the reaction contained 1.90 U/ml and 2.85 U/ml of ReSH and KlADH activities, respectively. (B) As the figure shows, the PH2 supplied to the reaction mixture was increased from 10% to 100%. The SF prepared from the cell suspension used for the reaction contained 1.86 U/ml and 3.61 U/ml of ReSH and KlADH activities, respectively.