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Figure 3 | Microbial Cell Factories

Figure 3

From: Hydrogen-driven asymmetric reduction of hydroxyacetone to (R)-1,2-propanediol by Ralstonia eutropha transformant expressing alcohol dehydrogenase from Kluyveromyces lactis

Figure 3

Hydrogen-driven fed-batch production of 1,2-propanediol by R. eutropha transformant H16-KLB4 with a jar fermenter. The reaction started with 120 ml of reaction mixture containing 60 ml cell suspension (1 g wet cell/5 ml reaction buffer) and 2% (v/v) hydroxyacetone. Hydroxyacetone was added periodically as indicated by arrows as the reaction proceeded. The SF prepared from the cell suspension used for the reaction contained 2.84 U/ml and 1.38 U/ml of ReSH and KlADH activities, respectively.

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Microbial Cell Factories

ISSN: 1475-2859

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