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Figure 2 | Microbial Cell Factories

Figure 2

From: Hydrogen-driven asymmetric reduction of hydroxyacetone to (R)-1,2-propanediol by Ralstonia eutropha transformant expressing alcohol dehydrogenase from Kluyveromyces lactis

Figure 2

Hydrogen-driven production of 1,2-propanediol by R. eutropha transformant H16-KLB4 using various amount of cell suspension. The initial concentration of hydroxyacetone (v/v) in the reaction mixture was 2% (â—‹), 3% (black triangle), or 5% (black square). The SF prepared from the cell suspension (1 g wet cell/5 ml reaction buffer) used for the reaction contained 2.25 U/ml and 1.73 U/ml of ReSH and KlADH activities, respectively. In each condition, reaction was performed in two vials.

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