Table 3 Bacterial strains and plasmids used in or constructed for this study
Strain or plasmid | Relevant properties† | Source or reference |
|---|---|---|
Strain | ||
Bacillus subtilis | ||
168 | trpC2 | [4] |
168∆rocG | trpC2 ∆rocG::spec | This study |
MGB874 | trpC2 ∆prophage1-6 ∆PBSX ∆SPβ ∆pks ∆skin ∆pps∆ (ydeK-ydhU) ∆(yisB-yitD) ∆(yunA-yurT) ∆(cgeE-yodU) ∆(ypqP-ypmQ) ∆(yeeK-yesX)∆(pdp-rocR) ∆(ycxB-sipU) ∆(yrkS-yraK) ∆(sboA-ywhH) ∆(yyb-yyaJ) ∆(yncM-yndN) | [6] |
874∆rocG | MGB874 ∆rocG::spec | [9] |
Escherichia coli | ||
HB101 | supE44 ∆(mcrC-mrr) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1 | Takara Bio |
Plasmid | ||
pHY300PLK | Shuttle vector for E. coli and B. subtilis | Takara Bio |
pHYS237 | pHY300PLK carrying the gene for alkaline endo-1,4-β-glucanase (Egl-237) from Bacillus sp. strain KSM-S237, containing amp and tet | [6] |