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Table 3 Bacterial strains and plasmids used in or constructed for this study

From: Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditions

Strain or plasmid Relevant properties Source or reference
Bacillus subtilis   
168 trpC2 [4]
168∆rocG trpC2rocG::spec This study
MGB874 trpC2 ∆prophage1-6 ∆PBSX ∆SPβ ∆pks ∆skin ∆pps∆ (ydeK-ydhU) ∆(yisB-yitD) ∆(yunA-yurT) ∆(cgeE-yodU) ∆(ypqP-ypmQ) ∆(yeeK-yesX)∆(pdp-rocR) ∆(ycxB-sipU) ∆(yrkS-yraK) ∆(sboA-ywhH) ∆(yyb-yyaJ) ∆(yncM-yndN) [6]
874∆rocG MGB874 ∆rocG::spec [9]
Escherichia coli   
HB101 supE44(mcrC-mrr) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1 Takara Bio
pHY300PLK Shuttle vector for E. coli and B. subtilis Takara Bio
pHYS237 pHY300PLK carrying the gene for alkaline endo-1,4-β-glucanase (Egl-237) from Bacillus sp. strain KSM-S237, containing amp and tet [6]
  1. Antibiotic resistance genes are abbreviated as follows: amp, ampicillin; tet, tetracycline; spec, spectinomycin; neo, neomycin.