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Table 3 Bacterial strains and plasmids used in or constructed for this study

From: Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditions

Strain or plasmid Relevant properties Source or reference
Strain   
Bacillus subtilis   
168 trpC2 [4]
168∆rocG trpC2rocG::spec This study
MGB874 trpC2 ∆prophage1-6 ∆PBSX ∆SPβ ∆pks ∆skin ∆pps∆ (ydeK-ydhU) ∆(yisB-yitD) ∆(yunA-yurT) ∆(cgeE-yodU) ∆(ypqP-ypmQ) ∆(yeeK-yesX)∆(pdp-rocR) ∆(ycxB-sipU) ∆(yrkS-yraK) ∆(sboA-ywhH) ∆(yyb-yyaJ) ∆(yncM-yndN) [6]
874∆rocG MGB874 ∆rocG::spec [9]
Escherichia coli   
HB101 supE44(mcrC-mrr) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1 Takara Bio
Plasmid   
pHY300PLK Shuttle vector for E. coli and B. subtilis Takara Bio
pHYS237 pHY300PLK carrying the gene for alkaline endo-1,4-β-glucanase (Egl-237) from Bacillus sp. strain KSM-S237, containing amp and tet [6]
  1. Antibiotic resistance genes are abbreviated as follows: amp, ampicillin; tet, tetracycline; spec, spectinomycin; neo, neomycin.