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Table 1 Genes up-regulated in 874ΔrocG cells (top-ranked 20 genes)

From: Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditions

Genea Producta Functiona Average signalb Ratioc Transcriptional factord
    MGB874 874ΔrocG   
nrgA ammonium transporter ammonium uptake 208 3926 18.91 TnrA(+)
yvrI co-sigma factor with YvrHa RNA polymerase sigma factor 92 1266 13.78 YvrH(+)
ykzB unknown unknown 92 1078 11.72 TnrA(+)
nasC nitrate reductase (catalytic subunit) utilization of nitrate 128 1463 11.46 GlnR(−), TnrA(+)
ansZ asparaginase asparagine utilization 202 1976 9.78 TnrA(+)
tnrA transcription activator/ repressor regulation of nitrogen assimilation 103 981 9.56 TnrA(+)
nasA nitrate transporter nitrate uptake 227 1975 8.7 GlnR(−), TnrA(+)
yvmB unknown unknown 119 977 8.23  
yjgD unknown survival of ethanol stress 158 1185 7.51  
nasB nitrate reductase (electron transfer subunit) utilization of nitrate 180 1346 7.49 GlnR(−), TnrA(+)
htrA serine protease Do protein quality control 719 4742 6.6 CssR(+), HtrA(−)
ygxB unknown unknown 184 1156 6.29  
yqzH unknown unknown 248 1463 5.9 LexA(−)
spoVFB dipicolinate synthase (subunit B) dipicolic acid production 82 475 5.8  
nrgB nitrogen-regulated PII-like protein regulation of ammonium uptake 821 4727 5.76 TnrA(+)
ntdA sugar aminotransferase synthesis of antibiotic neotrehalosadiamine 133 707 5.33 YhjM(+)
bmrU multidrug resistance protein multidrug resistance 180 949 5.28  
yrbD sodium/proton-dependent alanine transporter uptake of alanine 327 1721 5.27  
yitT unknown unknown 247 1301 5.26  
yuzA unknown unknown 146 758 5.2  
  1. aThe SubtiWiki was used as a reference for the genes, products and functions [21].
  2. bThe average signal intensities of probes in each coding sequence.
  3. cThe ratio of each of the genes was obtained by dividing the average signal intensity in each coding sequence of 874ΔrocG cells by that for MGB874 cells.
  4. dThe Database of Transcriptional Regulation in Bacillus subtilis (DBTBS) was used as a reference [22]. Transcriptional activators or repressors are indicated by a (+) or (−), respectively. TnrA is shown in bold type.