Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditions

Table 1 Genes up-regulated in 874ΔrocG cells (top-ranked 20 genes)

Gene^a	Product^a	Function^a	Average signal^b		Ratio^c	Transcriptional factor^d
			MGB874	874ΔrocG
nrgA	ammonium transporter	ammonium uptake	208	3926	18.91	TnrA(+)
yvrI	co-sigma factor with YvrHa	RNA polymerase sigma factor	92	1266	13.78	YvrH(+)
ykzB	unknown	unknown	92	1078	11.72	TnrA(+)
nasC	nitrate reductase (catalytic subunit)	utilization of nitrate	128	1463	11.46	GlnR(−), TnrA(+)
ansZ	asparaginase	asparagine utilization	202	1976	9.78	TnrA(+)
tnrA	transcription activator/ repressor	regulation of nitrogen assimilation	103	981	9.56	TnrA(+)
nasA	nitrate transporter	nitrate uptake	227	1975	8.7	GlnR(−), TnrA(+)
yvmB	unknown	unknown	119	977	8.23
yjgD	unknown	survival of ethanol stress	158	1185	7.51
nasB	nitrate reductase (electron transfer subunit)	utilization of nitrate	180	1346	7.49	GlnR(−), TnrA(+)
htrA	serine protease Do	protein quality control	719	4742	6.6	CssR(+), HtrA(−)
ygxB	unknown	unknown	184	1156	6.29
yqzH	unknown	unknown	248	1463	5.9	LexA(−)
spoVFB	dipicolinate synthase (subunit B)	dipicolic acid production	82	475	5.8
nrgB	nitrogen-regulated PII-like protein	regulation of ammonium uptake	821	4727	5.76	TnrA(+)
ntdA	sugar aminotransferase	synthesis of antibiotic neotrehalosadiamine	133	707	5.33	YhjM(+)
bmrU	multidrug resistance protein	multidrug resistance	180	949	5.28
yrbD	sodium/proton-dependent alanine transporter	uptake of alanine	327	1721	5.27
yitT	unknown	unknown	247	1301	5.26
yuzA	unknown	unknown	146	758	5.2

^aThe SubtiWiki was used as a reference for the genes, products and functions [21].
^bThe average signal intensities of probes in each coding sequence.
^cThe ratio of each of the genes was obtained by dividing the average signal intensity in each coding sequence of 874ΔrocG cells by that for MGB874 cells.
^dThe Database of Transcriptional Regulation in Bacillus subtilis (DBTBS) was used as a reference [22]. Transcriptional activators or repressors are indicated by a (+) or (−), respectively. TnrA is shown in bold type.

ISSN: 1475-2859