Figure 3From: Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditionsTime course of transcription during growth of B. subtilis strains MGB874 and 874ΔrocG. The strains MGB874 (open circles) and 874∆rocG (closed circles) were transformed with pHYS237. The transformants were cultured using shake-flask fermentation. Transcript levels for htrB, nrgA and gltA were determined by qRT-PCR (primers shown in Additional file 2: Table S1). Transcript levels were normalized to 16S rRNA levels. Error bars represent standard deviations (n=3).Back to article page