Figure 3

Loop out recombination. Through the highly recombinogenic nature of P. pastoris multiple copies of the vectors can integrate in a head-to-tail orientation. This will create repeat regions of homology which can recombine to remove either the whole or parts of the vector. For a two copy clone there is the potential for at least five loop out regions (based on the design of the vector) and this can increase to at least 11 for a three copy clone.