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Table 1 Strains and plasmids used in this study

From: SACE_5599, a putative regulatory protein, is involved in morphological differentiation and erythromycin production in Saccharopolyspora erythraea

Vector/strain name Description Promoter Gene Reference
S.erythraea WT- NRRL23338 / / [31, 32]
S.erythraea ABE1441 / / Acies Bio
pABE53 ɸC31, apramycin PermE* without RBS SACE_5599 (184 aa) This work
pABE101 ɸC31, apramycin PermE* with RBS SACE_5599 (184 aa) This work
pABE102 ɸC31, apramycin PermE* without RBS SACE_5599 (184 aa) HA-tag This work
pABE103 ɸC31, apramycin PermE* with RBS SACE_5599 (184 aa) HA-tag This work
pABE104 ɸC31, apramycin PermE* without RBS SACE_5599 (219 aa) This work
pABE105 ɸC31, apramycin PermE* with RBS SACE_5599 (219 aa) This work
pABE106 ɸC31, apramycin PermE* without RBS SACE_5599 (219 aa ) HA-tag This work
pABE107 ɸC31, apramycin PermE* with RBS SACE_5599 (219 aa) HA-tag This work
pABE110 Suicide, apramycin / 344 bp long part of the SACE_5599 gene for disruption This work
pABE21 ɸC31, apramycin PermE* without RBS bldD gene This work
pABE112 ɸC31, thiostrepton PermE* without RBS SACE_5599 (219 aa ) HA-tag This work
  1. Site-specific integration with bacteriophage ɸC31 integrase is indicated for pSET152-derived plasmids; “/” refers to “none or not applicable”.