Table 1 Strains and plasmids used in this study
Vector/strain name | Description | Promoter | Gene | Reference |
|---|---|---|---|---|
S.erythraea | WT- NRRL23338 | / | / | |
S.erythraea | ABE1441 | / | / | Acies Bio |
pABE53 | ɸC31, apramycin | PermE* without RBS | SACE_5599 (184 aa) | This work |
pABE101 | ɸC31, apramycin | PermE* with RBS | SACE_5599 (184 aa) | This work |
pABE102 | ɸC31, apramycin | PermE* without RBS | SACE_5599 (184 aa) HA-tag | This work |
pABE103 | ɸC31, apramycin | PermE* with RBS | SACE_5599 (184 aa) HA-tag | This work |
pABE104 | ɸC31, apramycin | PermE* without RBS | SACE_5599 (219 aa) | This work |
pABE105 | ɸC31, apramycin | PermE* with RBS | SACE_5599 (219 aa) | This work |
pABE106 | ɸC31, apramycin | PermE* without RBS | SACE_5599 (219 aa ) HA-tag | This work |
pABE107 | ɸC31, apramycin | PermE* with RBS | SACE_5599 (219 aa) HA-tag | This work |
pABE110 | Suicide, apramycin | / | 344 bp long part of the SACE_5599 gene for disruption | This work |
pABE21 | ɸC31, apramycin | PermE* without RBS | bldD gene | This work |
pABE112 | ɸC31, thiostrepton | PermE* without RBS | SACE_5599 (219 aa ) HA-tag | This work |