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Figure 4 | Microbial Cell Factories

Figure 4

From: A moth pheromone brewery: production of (Z)-11-hexadecenol by heterologous co-expression of two biosynthetic genes from a noctuid moth in a yeast cell factory

Figure 4

Gas chromatography of oxidized yeast extracts. (A) When stimulated with the oxidized extract from Ase∆11/CUP1p-AseFAR/GAL1p-pYEXCHT the H. virescens male antenna responds to the pheromone components Z9-14:Al and Z11-16:Al, as well as to 14:Al, Z9-16:Al and to a lesser extent to 16:Al. (B) In the negative control there are no aldehydes and no antennal responses to the oxidized yeast extract at corresponding retention times (dashed lines), or at any other elution time. Flame ionization detector (FID) response is marked in black, while electroantennographic detector (EAD) response using a male H. virescens antenna is marked in blue. Repeated experiments produced the same EAD response pattern.

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