Figure 4

Time course of extracellular α-amylase production by Brevibacillus choshinensis SP3 at different temperatures. A) α-amylase activity of culture supernatant of Brevibacillus carrying α-amylase-pNC and SEC-α-amylase-pHis1522 grown at 37°C (red line), 30°C (green line), 25°C (blue line) B) Western blot of the of culture supernatant of Brevibacillus carrying α-amylase-pNC and SEC-α-amylase-pHis1522 grown at 25°C.The α-amylase-pHis1522 was induced with 0,5% of xylose. All cultures were grown in triplicate, and each experiment was performed at least twice. Error bars indicate standard deviations.