|
Protein
|
Growth conditions
|
Biomass at the harvest timea(g/L)
|
Pure chimeric proteinb (mg/L)
|
Pure mature protein (mg/L)
|
|---|
|
Trx-TB10.4
|
Flasks
|
4.8
|
12
|
4c
|
| |
Bioreactor
|
8.5
|
80
|
25c
|
|
Trx-Ag85B
|
Flasks
|
10.5
|
9
|
4.7c
|
| |
Bioreactor
|
19.5
|
30
|
16c
|
|
His-full 2
|
Flasks
|
6.5
|
/
|
0.5
|
| |
Bioreactor
|
6.5
|
/
|
2.5
|
- agrams of E. coli cells (wet weight) per liter of cultivation broth at the harvest time.
- bmilligrams of protein obtained upon purification by nickel-affinity chromatography per liter of cultivation broth.
- cmilligrams of protein obtained upon proteolytic cleavage and separation by nickel-affinity chromatography per liter of cultivation broth.
