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Table 2 Production yield of purified recombinant Trx-TB10.4, Trx-Ag85B (chimeric and single antigens, after proteolytic cleavage), and His- full 2 by E. coli cells grown at flask and bioreactor scales

From: Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins

Protein Growth conditions Biomass at the harvest timea(g/L) Pure chimeric proteinb (mg/L) Pure mature protein (mg/L)
Trx-TB10.4 Flasks 4.8 12 4c
  Bioreactor 8.5 80 25c
Trx-Ag85B Flasks 10.5 9 4.7c
  Bioreactor 19.5 30 16c
His-full 2 Flasks 6.5 / 0.5
  Bioreactor 6.5 / 2.5
  1. agrams of E. coli cells (wet weight) per liter of cultivation broth at the harvest time.
  2. bmilligrams of protein obtained upon purification by nickel-affinity chromatography per liter of cultivation broth.
  3. cmilligrams of protein obtained upon proteolytic cleavage and separation by nickel-affinity chromatography per liter of cultivation broth.