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Table 2 Catalytic efficiency of NS3 produced at various temperatures

From: Over-expression and characterization of NS3 and NS5A of Hepatitis C virus genotype 3a

Protein

Temperature

Kcat(s-1)

Kcat/Km

Kcat/Km(μM-1 · s-1)

(μM-1 · s-1)

× Purification yield (mg/L1)

NS3 (0.1% OPG)

32°C

3.17 ± 0.10

2.37

5.93

25°C

2.27 ± 0.07

1.77

7.04

14°C

1.836 ± 0.11

1.55

6.20

NS3 (0.4% OPG)

32°C

1.840 ± 0.05

5.93

14.82

25°C

1.485 ± 0.04

4.22

16.88

14°C

1.176 ± 0.03

3.28

13.12

  1. 1The yield of purified proteins were measured milligram per liter of culture (Expression medium).
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Microbial Cell Factories

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