Table 2 Strains and plasmids used in this study
Strains or plasmids | Genotype or characteristic | Resources or references |
|---|---|---|
E. coli Strains | Â | Â |
DH5α | F-, φ80d/lacZ∆M15, ∆(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 | TaKaRa |
DH10B | F-,mcr A, ∆(mrr-hsd RMS-mcr BC), φ80lac Z∆M15, ∆lac X74, rec A1, end A1, ara D139, ∆ (ara, leu)7697, gal U, gal K, λ-, rps L, nup G/pMON14272/pMON7124 | Invitrogen |
DH1 | F-, glnV44(AS), λ-, rfbC1, gyrA96(NalR), recA1, endA1, thi-1, hsdR17 | CGSC |
(No. 6040) | ||
MG1655 | F-, λ-, rph-1 | CGSC |
(No. 6300) | ||
BW25113 | rrnB3, ∆lacZ4787, hsdR514, ∆(araBAD)567, ∆(rhaBAD)568 rph-1 | CGSC |
(No. 7636) | ||
Bl21(DE3) | F–, dcm, ompT, hsdS(rB–, mB–), gal, λ(DE3) | Novagen |
YJE005 | DH5α/pTrc99A-gldA-nox | This study |
YJE006 | DH5α/pTrc99A-gldA-nox + ntt4 | This study |
Plasmids | Â | Â |
pMD18-T | lacZ, pBR322 ori, bla, cloning vector | TaKaRa |
pTrc99A | lacI, pBR322 ori, bla, expression vector | Amersham Pharmacia |
pET15K-ntt4 | ntt4 inserted within Nde I and Bam H I sites, kan | [11] |
pBCTC-ntt4 | ntt4 inserted within Sac I and Bam H I sites, kan | This study |
pBCTD-ntt4 | ntt4 inserted within Sac I and Bam H I sites, kan | This study |
pTrc99A-gldA-nox | gldA and nox transcription under Trc promoter | This study |
pTrc99A-gldA-nox + ntt4 | gldA and nox transcription under Trc promoter, ntt4 under gntT105p promoter. | This study |