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Table 2 Strains and plasmids used in this study

From: Engineering NAD+ availability for Escherichia coli whole-cell biocatalysis: a case study for dihydroxyacetone production

Strains or plasmids Genotype or characteristic Resources or references
E. coli Strains   
DH5α F-, φ80d/lacZ∆M15,(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 TaKaRa
DH10B F-,mcr A, ∆(mrr-hsd RMS-mcr BC), φ80lac Z∆M15, ∆lac X74, rec A1, end A1, ara D139, ∆ (ara, leu)7697, gal U, gal K, λ-, rps L, nup G/pMON14272/pMON7124 Invitrogen
DH1 F-, glnV44(AS), λ-, rfbC1, gyrA96(NalR), recA1, endA1, thi-1, hsdR17 CGSC
(No. 6040)
MG1655 F-, λ-, rph-1 CGSC
(No. 6300)
BW25113 rrnB3,lacZ4787, hsdR514,(araBAD)567,(rhaBAD)568 rph-1 CGSC
(No. 7636)
Bl21(DE3) F–, dcm, ompT, hsdS(rB, mB), gal, λ(DE3) Novagen
YJE005 DH5α/pTrc99A-gldA-nox This study
YJE006 DH5α/pTrc99A-gldA-nox + ntt4 This study
Plasmids   
pMD18-T lacZ, pBR322 ori, bla, cloning vector TaKaRa
pTrc99A lacI, pBR322 ori, bla, expression vector Amersham Pharmacia
pET15K-ntt4 ntt4 inserted within Nde I and Bam H I sites, kan [11]
pBCTC-ntt4 ntt4 inserted within Sac I and Bam H I sites, kan This study
pBCTD-ntt4 ntt4 inserted within Sac I and Bam H I sites, kan This study
pTrc99A-gldA-nox gldA and nox transcription under Trc promoter This study
pTrc99A-gldA-nox + ntt4 gldA and nox transcription under Trc promoter, ntt4 under gntT105p promoter. This study