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E. coli
Strains
| | |
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DH5α
|
F-, φ80d/lacZ∆M15, ∆(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1
|
TaKaRa
|
|
DH10B
|
F-,mcr A, ∆(mrr-hsd RMS-mcr BC), φ80lac Z∆M15, ∆lac X74, rec A1, end A1, ara D139, ∆ (ara, leu)7697, gal U, gal K, λ-, rps L, nup G/pMON14272/pMON7124
|
Invitrogen
|
|
DH1
|
F-, glnV44(AS), λ-, rfbC1, gyrA96(NalR), recA1, endA1, thi-1, hsdR17
|
CGSC
|
|
(No. 6040)
|
|
MG1655
|
F-, λ-, rph-1
|
CGSC
|
|
(No. 6300)
|
|
BW25113
|
rrnB3, ∆lacZ4787, hsdR514, ∆(araBAD)567, ∆(rhaBAD)568 rph-1
|
CGSC
|
|
(No. 7636)
|
|
Bl21(DE3)
|
F–, dcm, ompT, hsdS(rB–, mB–), gal, λ(DE3)
|
Novagen
|
|
YJE005
|
DH5α/pTrc99A-gldA-nox
|
This study
|
|
YJE006
|
DH5α/pTrc99A-gldA-nox + ntt4
|
This study
|
|
Plasmids
| | |
|
pMD18-T
|
lacZ, pBR322 ori, bla, cloning vector
|
TaKaRa
|
|
pTrc99A
|
lacI, pBR322 ori, bla, expression vector
|
Amersham Pharmacia
|
|
pET15K-ntt4
|
ntt4 inserted within Nde I and Bam H I sites, kan
|
[11]
|
|
pBCTC-ntt4
|
ntt4 inserted within Sac I and Bam H I sites, kan
|
This study
|
|
pBCTD-ntt4
|
ntt4 inserted within Sac I and Bam H I sites, kan
|
This study
|
|
pTrc99A-gldA-nox
|
gldA and nox transcription under Trc promoter
|
This study
|
|
pTrc99A-gldA-nox + ntt4
|
gldA and nox transcription under Trc promoter, ntt4 under gntT105p promoter.
|
This study
|
