From: Engineering NAD+ availability for Escherichia coli whole-cell biocatalysis: a case study for dihydroxyacetone production
Plasmid
Promoter
Cellular NAD(H) level (mM)b
Increment
Empty vector
NTT4 expression
pET15k
T7
1.72 ± 0.03
1.85 ± 0.01
7.9%
pBCTC
gap P1
1.96 ± 0.00
2.13 ± 0.01
8.3%
pBCTD
gntT105 P
1.86 ± 0.05
4.47 ± 0.14
141.0%