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Table 1 Intracellular NAD(H) levels of E. coli DH5α cells a harboring NTT4 expression and corresponding empty plasmids

From: Engineering NAD+ availability for Escherichia coli whole-cell biocatalysis: a case study for dihydroxyacetone production

Plasmid Promoter Cellular NAD(H) level (mM)b Increment
Empty vector NTT4 expression
pET15k T7 1.72 ± 0.03 1.85 ± 0.01 7.9%
pBCTC gap P1 1.96 ± 0.00 2.13 ± 0.01 8.3%
pBCTD gntT105 P 1.86 ± 0.05 4.47 ± 0.14 141.0%
  1. aCells were cultivated at 37°C for 12 h in LB media containing 0.2 mM NAD+, collected by centrifugation and washed twice with PBS buffer.
  2. bAll data represent the averages ± SDs for three independent samples.