A novel multigene expression construct for modification of glycerol metabolism in Yarrowia lipolytica

Table 3 Quantitative comparison of cultivation parameters obtained in shake flask cultivations aiming at 2-PE production

	2-PE	2-PE	Utilized L-Phe	2-PE/L-Phe	2-PE/biomass	OD₆₀₀
	[mg L^-1]	[mg L^-1 h^-1]	[g L^-1]	[g_2-PEg_L-Phe ^-1]	[g OD₆₀₀ ^-1]	[OD₆₀₀units]
NCYC3825	1056.0	4.7	2.57	0.41	0.19	5.29
A18	320.6	1.68	0.99	0.32	0.17	1.88

Final titer [mg L^-1], volumetric productivity [mg L^-1 h^-1], utilized L-Phe [g L^-1], yield 2-PE from L-Phe [g_2-PE g_L-Phe ^-1], biomass normalized 2-PE titer 2-PE/biomass [g OD₆₀₀ ^-1], biomass production expressed as turbidity at 600 nm wavelength [OD₆₀₀ units]. Experiments were carried out in triplicate. Statistical importance of the observed difference was analyzed with t-Student’s test (MS Excel AnalysisToolPack) – the observed differences were statistically important at p < 0.05. For kinetics presentation see (Figure 8).

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